Journal: Frontiers in Microbiology
Article Title: Exploration of the role of the penicillin binding protein 2c (Pbp2c) in inducible β-lactam resistance in Corynebacteriaceae
doi: 10.3389/fmicb.2024.1327723
Figure Lengend Snippet: Induction of meropenem resistance by the disk diffusion assay for strain C. jeikeium K411. Plates were incubated for 48 h. The assays were performed on BHI agar containing 0.5% Tween 80 and meropenem at 128 μg/mL. The disks contained 1 : ampicillin (10 μg), 2: oxacillin (5 μg), 3: piperacillin (30 μg), 4: cephalothin (30 μg), 5: cefoxitin (30 μg), 6: ceftazidime (30 μg), 7: cefepime (30 μg), 8: colistin (30 μg), 9: chloramphenicol (30 μg), 10: benzylpenicillin (10 U), 11: amoxicillin (25 μg), 12: amoxicillin/clavulanate (20 μg/10 μg), 13: gentamicin (10 μg), 14: erythromycin (15 μg), 15: norfloxacin (10 μg), 16: vancomycin (5 μg), 17: teicoplanin (30 μg), 18: tetracycline (30 μg), 19: imipenem (10 μg), 20: meropenem (10 μg), or 21: ertapenem (10 μg).
Article Snippet: Antibiotics used for selection were ampicillin (Ap) at 100 μg/mL (Euromedex, Souffelweyersheim, France), kanamycin (Km) at 50 μg/mL (Duchefa, Haarlem, The Netherlands), and chloramphenicol (Cm) at 30 μg/mL for E. coli and 10 μg/mL for C. glutamicum (Duchefa).
Techniques: Diffusion-based Assay, Incubation